Tangential Flow Depth Filtration® Technology (TFDF®) for Bioreactor Perfusion
New! Combines the Advantages of Tangential Flow Filtration and Depth Filtration to Minimize Membrane Fouling and Maximize Protein Passage

Hollow Fiber Structure allows cells to be swept across the filter surface and back into the bioreactor

Thick Fiber Wall and large pore size distribution acts a depth filter to trap cellular debris and minimize membrane fouling

  • Minimize Filter Fouling
  • Maximize Protein Passage
  • Compatible with Micro-Carrier and Suspension Cell Cultures
  • Provided in Gamma Sterilized Single-Use Perfusion Flow Paths
  • Scalable for 1.5L to 2000L Bioreactors
  • 100% Passage of Target Proteins
New TFDF Module

One of the most problematic areas for filtration based cell perfusion is decreased protein sieving due to filter fouling. SpectrumLabs.com’s new Tangential Flow Depth Filtration® (TFDF®) technology overcomes these hurdles by combining the advantages of tangential flow filtration (TFF) with the advantages of depth filtration. As in standard hollow fiber filters using TFF, cells are pumped through the lumen of the fibers, sweeping them off the membrane back to the reactor. However, instead of the protein and cell debris forming a fouling gel layer at the inner diameter of the fiber, the wall adds a depth filtration portion that traps the debris inside the wall structure. This depth filtration aspect of the filter has a much greater dirt capacity than traditional hollow fiber TFF, thus enabling increased volumetric throughput while maintaining close to 100% passage of typical target proteins.

TFDF graphs

(Data generated by Keck Graduate Institute Team Master's Project)

TFDF technology uses both a large lumen ID, as well as large membrane pore distribution throughout the thickness of the fiber wall. TFDF is provided in a sterile Pro-Connex® Flow Path strictly for Spectrum's simple to use, low pressure, ready-to-operate KML® 100 and KPS® 600 TFF perfusion systems. This technology has also been shown to work with continuous bioprocessing platforms for suspension cells as well as microcarriers and adherent cells for passing viruses / virus-like particles (VLP).

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