KrosFlo® Perfusion Systems
Designed for Lab, Pilot and Production Scale Perfusion Operations
KMLTM 100 System for 1.5-100 Bioreactor Volumes
KM 100 System for 1.5-100L Bioreactor Volumes

KML [KrosFlo MagLev] Benchtop Perfusion and Tangential Flow Filtration (TFF) System is designed for many TFF process operations. With perfusion, the KML is designed for managing 1.5 L to 100L working volume bioreactors using gamma sterilized single-use perfusion flow-paths (MBT's). The system uses a single-use, non-pulsating, levitating magnetic pump controlled by the PLC. The pump is low shear for improved cell viability. The KML system has a small benchtop footprint and comes with a detachable flow path stand.

The KML is a completely integrated system with a color touchscreen to display pressures, flows, weights and process overview with end user set-point functionality. There are visual and audible alarms with safety interlocks to ensure process parameters are maintained. Operators are able to input values for managing six (6) different TFF modes and a manual operation mode. The system comes with two (2) auxiliary peristaltic pumps and two (2) auxiliary scales for precise the management of the flow dynamics are sold separately. Scale Sizes are selected by the user. The non-invasive components include a retentate control valve that manages TMP by adjusting the pinch distance between the plunger and the tubing, and an ultrasonic retentate flow meter that clamps on the tubing. To prevent cells from settling and forming a gel layer on the membrane lumen, the KML system adjusts through a PID control loop from the flow meter.

Further advancements include automatic back pulsing features, USB port for real time data collection, as well as graphical display of process data. KML modes of operation include Perfusion Mode and 5 traditional TFF modes:

  1. Concentration
  2. Concentration/Diafiltration
  3. Concentration/Diafiltration/Final Concentration
  4. Concentration/Diafiltration(1)/Diafiltration (2)/Final Concentration
  5. Continuous Feed or Diafiltration
KML 100 Advantages

Low Shear: Improved Cell Viability for lengthy perfusion operations

Small Benchtop Footprint (20" tall x 19" wide x 16" deep) / (50.8cm tall x 48.3cm wide x 40.6cm deep)

Completely Integrated with the Ethernet Communication Option

Automated auxiliary pumps (two included; 3rd is optional)

Built-in Color Touch Screen for easy operational interface

Non-invasive Automated Retentate Control Valve

Non-invasive Ultrasonic Clamp-on Retentate Flow Meter

USB port for Data Collection

Visual and Audible Alarms with Safety Interlocks

Versatile - can be used for TFF Perfusion, 5 additional TFF mode of operations and manual mode

KML 100 System Components

Magnetic Levitation Recirculation Pump

Flow Range 350 ml/min to 13 LPM at 7000 RPM @ 15 psig (1 bar)
0-9000 RPM range with 300 watt power supply
FDA approved materials, USP Class VI, Animal BSE/TSE Free Material
PLC with color 8.4" touchscreen HMI
0-40° C Operating Range
CE/UL/FCC approved
Automation Direct DL06 PLC with 800x600 Resolution HMI
Splash-proof

Integrated Detachable Hollow Fiber Flow Path Stand

2 Integrated Peristaltic Pumps

Flow ranges from 0.16 to 347 ml/min
Permeate pump sequenced for back-pulsing in the Perfusion mode
RS232 communication
Integrated power within single connection to KML

Retentate Control Valve

Non-invasive to process stream
4-20 mA control

Ultrasonic Single Pass Retentate Flow Meter

Measures flow up to 20LPM +/- 5%
Calibrated for Dow Pharma tubing at 35°C

Designed for Use with Hollow Fiber Filter Modules

MiniKros Sampler Modules (S02, S04 and S06 series)
MiniKros Modules (N02, N04 and N06 series)
500 - 8500 cm2 hollow fiber surface area range

KMLTM 200 System for 1.5-100 Bioreactor Volumes
KPS 200 System for 1.5-100L Bioreactor Volumes

Spectrum has developed two KrosFlo Perfusion Systems, the KPS 200 and the KPS 600. The KPS 200 system can be used with 1.5 -100L liter bioreactors. The KPS 600 can be used with 100 - 2000 liter bioreactors. The KPS Systems using Spectrum's sterilized perfusion flow-path assemblies will automatically control and maintain critical operational parameters defined by the operator (Recirculation Shear Rate, TMP control, Permeate Flow/VVD and Reactor Volume Maintenance). Controls are performed without mechanical components contacting product. Traditional stainless systems need to be CIP or SIP prior to use while the KPS 200 and KPS 600 are simply loaded with the sterile flow-path assemblies required for the tangential flow perfusion operation. Robust integrated solutions and associated connections minimizes the hold up volume and provides a completely drainable process loop for individual filters ranging with surface areas from 0.047 m2 to 1.0 m2 for the KPS 200 and 0.78 m2 to 12.5 m2 for the KPS 600.

Kros ® Perfusion System Components

System components include:

Software: Proficy HMI/SCADA-IFIX

Ethernet port for connection to central data acquisition system

15" Color Touch Screen Computer/Display

Sequences Available in Semi-Automated or Automated

Ultrasonic Non-Invasive Retentate Flow Meter (Permeate Flow Meter optional)

Nema 4X Rated Enclosure

Controller Displayed reading and renderings of the flow-path

Visual and Audible Alarms with safety interlocks

Retentate Control Valve that is non-invasive to the process stream

USB port for connection flash drive to transfer data

USB port can be used for connecting an external mouse or keyboard

KPS 200 System Chart
KMLTM 600 System for 100 - 2000 Bioreactor Volumes
KM 600 System for 100 - 2000 L Bioreactor Volumes

Spectrum has developed two KrosFlo Perfusion Systems, the KPS 200 and the KPS 600. The KPS 200 system can be used with 1.5 -100L liter bioreactors. The KPS 600 can be used with 100 - 2000 liter bioreactors. The KPS Systems using Spectrum's sterilized perfusion flow-path assemblies will automatically control and maintain critical operational parameters defined by the operator (Recirculation Shear Rate, TMP control, Permeate Flow/VVD and Reactor Volume Maintenance). Controls are performed without mechanical components contacting product. Traditional stainless systems need to be CIP or SIP prior to use while the KPS 200 and KPS 600 are simply loaded with the sterile flow-path assemblies required for the tangential flow perfusion operation. Robust integrated solutions and associated connections minimizes the hold up volume and provides a completely drainable process loop for individual filters ranging with surface areas from 0.047 m2 to 1.0 m2 for the KPS 200 and 0.78 m2 to 12.5 m2 for the KPS 600.

Kros ® Perfusion System Components

System components include:

Software: Proficy HMI/SCADA-IFIX

Ethernet port for connection to central data acquisition system

15" Color Touch Screen Computer/Display

Sequences Available in Semi-Automated or Automated

Ultrasonic Non-Invasive Retentate Flow Meter (Permeate Flow Meter optional)

Nema 4X Rated Enclosure

Controller Displayed reading and renderings of the flow-path

Visual and Audible Alarms with safety interlocks

Retentate Control Valve that is non-invasive to the process stream

USB port for connection flash drive to transfer data

USB port can be used for connecting an external mouse or keyboard

for more information:

call Customer Service at (800) 634-3300 (toll-free US and Canada) • (310) 885-4600 (world-wide) email us at customerservice@spectrumlabs.com or submit the form by clicking on the request info button

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