Ion Exchange Resins

Spectrum manufactures a complete range of Ion Exchange Resins for preparative scale ion exchange chromatography. Spectra/Gel® Ion Exchange Resins are ideally suited for protein purification, antibody isolation, and peptide fractionation. They are also used in traditional inorganic ion exchange.

Spectra/Gel Ion Exchange Resins are durable, insoluble, and compatible with most aqueous and organic solvents. The structural polymeric backbone of the resin is styrene cross-linked with 2 to 8% divinylbenzene. The amount of cross-linkage determines both the pore size of the media and the capacity of the resin. (The pore size can be significant when separating proteins but is of little significance when doing inorganic separations.)

The type of exchange performed (cation or anion) is determined by functional groups added to the resins.

The resins are available in 3 different bead sizes. The medium (75-150 µm diameter) and large (150-300 µm diameter) beads are most useful for gravity flow and batch (non-flow) applications. The larger size of the beads makes for ready flow and easy separation from a bulk liquid. The medium (75-150 µm diameter) and small (40-75 µm diameter) beads are most useful for pumped applications. The smaller size of the beads reduces the chromatography plate height providing a more rapid equilibration at higher flow rates.

These resins are available with 3 different levels of cross-linkage in the polystyrene backbone. The greater the cross-linkage the greater the capacity of the resin and the smaller the pore size. For inorganic ion exchange the pore size is usually of little interest. For globular proteins, the approximate exclusion limits are 3000 Daltons at 2%, 1500 Daltons at 4% and 1000 Daltons at 8% cross-linkage.

Anion Exchange Resins
Spectra/Gel Anion Exchange Resins are strong (type 1) ion exchange resins. They use a trimethylbenzylammonium group as the exchange site. They are supplied in the chloride form (with a chloride ion occupying the exchange site). This form is very stable and has an excellent shelf life.

Anion Exchange Resin Characteristics

Type:
strong base anion exchanger
Active Group:
trimethylbenzylammonium
% divinylbenzene:
2%, 4%, or 8%
Supplied Ionic Form:
Cl-
Moisture Content:
43% to 48%
Volume Change:
Cl- to OH- is +20%
pH Range:
0 to 14
Selectivity:
Cl-/OH- is about 25
Order of Selectivity:
I > NO3 > Br > Cl > acetate > OH > F

Product No. Description Bread Size Capacity DVB Qty Price
122045 IE 1X2 Resin 40 to 75 µm (200 to 400 mesh) 0.6 meq/ml 2% 0.5 kg
122050 IE 1X2 Resin 75 to 150 µm (100 to 200 mesh) 0.6 meq/ml 2% 0.5 kg
122055 IE 1X2 Resin 150 to 300 µm (50 to 100 mesh) 0.6 meq/ml 2% 0.5 kg
122060 IE 1X4 Resin 40 to 75 µm (200 to 400 mesh) 1.0 meq/ml 4% 0.5 kg
122080 IE 1X8 Resin 75 to 150 µm (100 to 200 mesh) 1.2 meq/ml 8% 0.5 kg
122085 IE 1X8 Resin 150 to 300 µm (50 to 100 mesh) 1.2 meq/ml 8% 0.5 kg
Cation Exchange Resins
Spectra/Gel Cation Exchange Resins are strong acid (type 50) ion exchange resins. They contain a sulfonic acid active group as the exchange site. They are supplied with a hydrogen ion occupying the exchange site.
Cation Exchange Resin Characteristics
Type:
strong base anion exchanger
Active Group:
sulfonic acid
% divinylbenzene:
2%, 4%, or 8%
Supplied Ionic Form:
H+
Moisture Content:
51% to 54%
Volume Change:
NA+ to H+ is +8%
pH Range:
0 to 14
Selectivity:
NA+/H+ is about 1.5
Order of Selectivity:
Ba++ > Rb++ > Ca++ > Mg++ > Be++ > Ag+ > Cs+ > Rb+ > K+ > NH4+ > Na+ > H+ > Li+
Product No. Description Bead Size Capacity DVB Qty Price
122000 IE 50X2 Resin 50 to 75 µm (200 to 400 mesh) 0.6 meq/ml 2% 0.5 kg
122005 IE 50X2 Resin 75 to 150 µm (100 to 200 mesh) 0.6 meq/ml 2% 0.5 kg
122010 IE 50X2 Resin 150 to 300 µm (50 to 100 mesh) 0.6 meq/ml 2% 0.5 kg
122015 IE 50X4 Resin 40 to 75 µm (200 to 400 mesh) 1.2 meq/ml 4% 0.5 kg
122025 IE 50X4 Resin 150 to 300 µm (50 to 100 mesh) 1.2 meq/ml 4% 0.5 kg
122030 IE 50X8 Resin 40 to 75 µm (200 to 400 mesh) 1.7 meq/ml 8% 0.5 kg

Web Discount GIF
Disposable Columns
Disposable Columns

Our disposable columns can be filled with these ion exchange resins to create small disposable exchange columns..

Aqueous Columns
Our standard aqueous chromatography columns can also be used with these resins for cleaning larger samples.

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